Abstract Background CRISPR-based programmable transcriptional activators (PTAs) are used in plants for rewiring gene networks. Better tuning of their activity in a time and dose-dependent manner should allow precise control of gene expression. Here. we report the optimization of a Copper Inducible system called CI-switch for conditional gene activation in Nicotiana benthamiana. https://balboasurfandsstylers.shop/product-category/womens-sunshirt/